Sendai virus reprogramming efficiency in human dermal fibroblasts

Answer

No single source-reported figure answers the question. Printed Sendai efficiencies in human fibroblasts span a wide range, from about 8 colonies at day 21 (per a fixed cell number given in the record) 6cc1c5dd and 0.01-0.04% a264ba4c, through 0.04% for one patient line 106705d8, about 0.05% 64f33bfb, 0.076% as a pooled mean with SD 0.170 d521ea8d and 0.077% 32360b78, to 0.2% and 0.95% in two donors c708ffda, 0.33% to 0.64% 043097a8, 0.6% to 0.8% e456c6f0, 0.73% c1bfe5af and 1.37% 9d57c067. They differ in denominator, marker, matrix and scoring day, so they are not one quantity. The lowest printed value is the Vdovin abstract figure; the 0.04% used in some comparisons below is the top of MacArthur's printed 0.01-0.04% range, not the floor of the corpus.

Summary

The platform flags four conflicts, and none is a same-condition pair. First, 0.04% 36216bc8 against 0.73% c1bfe5af; second, 0.04% 36216bc8 against about 1% ee75dd7b; third, 0.04% 36216bc8 against 1.37% 9d57c067; fourth, 0.73% c1bfe5af against 1.37% 9d57c067. The 0.04% is the top of a printed 0.01-0.04% range for feeder-free neonatal fibroblasts by AP staining on day 25, with no stated denominator a264ba4c; the 0.73% divides AP-positive colonies by the cells replated on vitronectin after day 7 c1bfe5af; the 'around 1%' is the best configuration of in-house temperature-sensitive vectors on MEF feeders, not the kit, per infected cell seeded 3d70a66e; and the 1.37% is AP-positive colonies per cell number used on Geltrex in E8, up to day 25 9d57c067. The corpus does not settle whether any residual gap is biological.

Response to the peer objection: two facts earlier set aside are now used. Vdovin 2015 reports about 8 colonies at day 21 with Tra1-60-expressing colonies, against about 200 for episomal plasmids; the denominator is a fixed cell number stated in an abstract-only record, whether plated or infected cells is not stated, and the counting criterion is not stated 6cc1c5dd. This is the lowest Sendai fibroblast figure in the corpus, so the range floor above is corrected. Kuebler 2026 prints a Sendai-on-fibroblast mean of 0.076% of cells seeded with SD 0.170, a pooled value over many lines whose spread exceeds the mean, so a few high lines drive it; counting day and marker are not stated and supplementary files were not read d521ea8d. The spread is informative for a range question and is kept. The oxygen absence is re-checked: Kuebler states 5% O2 only for its RNA protocol and not for the Sendai arm, Schlaeger likewise only for mRNA, and Vdovin's abstract is silent, so oxygen remains unstated for every Sendai arm d521ea8d 32360b78.

Evidence

• Vdovin et al. 2015 - adult skin fibroblasts, about 8 colonies at day 21 versus about 200 for episomal; abstract only. 6cc1c5dd
• Manzini et al. 2015 - Sendai in three patients, the highest line 0.04%; cells transduced; MEF feeders; day 29. 106705d8
• Trevisan et al. 2017 - BJ fibroblasts, about 0.05% of transduced cells, TRA-1-60 colonies, n=3. 64f33bfb; a second record of the same paper repeats the same figure
• Schlaeger et al. 2015 - 0.077% as the mean of successful experiments per starting cell, n=9. 32360b78
• Kuebler et al. 2026 - fibroblast-derived lines in one laboratory, Sendai mean 0.076% ± 0.170, denominator seeded somatic cells. d521ea8d
• Elanzew et al. 2020 - 0.6 and 0.8% at day 21 on Geltrex; denominator and marker not stated. e456c6f0
• Pisal et al. 2018 - one donor, 550 AP-positive colonies, 0.73%, day 25. c1bfe5af
• Huber et al. 2019 - 1.37% ± 0.4 for Sendai, AP-positive colonies up to day 25, n=9. 9d57c067
• MacArthur et al. 2012 - between 0.01 and 0.04% by AP staining, feeder-free. 36216bc8 a264ba4c
• Fusaki et al. 2009 - research vectors, around 1%, ALP-positive colonies per infected cell seeded. 3d70a66e ee75dd7b
• Gerami-Naini 2016 043097a8 (six foot-skin lines, 0.33% to 0.64%), Castro-Vinuelas 2020 c708ffda (two donors, 0.2% and 0.95%), centenarian study 2019 5b3097c5 (0.02-0.06% with centrifugation, none without), Vashistha 2026 e968af3c and Kudva 2012 f769a092 (Sendai values only in figures; no number used).

Methodology

All eighteen verified facts are accounted for; records of one paper were counted once. Figures were grouped by denominator and marker because those decide whether two percentages are the same quantity. Printed ranges were kept as ranges and nothing was converted or averaged. The hypothesis is carried unchanged from the earlier version.

Limitations

• The denominator is not stated in several sources, and the scoring day is a window or unstated in most, so no two figures are matched e456c6f0 043097a8 c708ffda.
• Oxygen is stated for the Sendai arm in none of the sources.
• Several fibroblasts are neonatal or foot-skin, not adult dermal biopsy 64f33bfb 043097a8.
• Pisal is one donor c1bfe5af.
• Vdovin is abstract-only and Kuebler is a pooled mean with a large spread, so neither fixes a comparable value 6cc1c5dd d521ea8d.