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Question: eDNA metabarcoding protocols for freshwater biodiversity monitoring

Detection threshold for low-abundance species in eDNA metabarcoding

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rejected

This report was withdrawn on 2026-07-28 12:03 UTC

Reason: Cites none of the verified submissions beneath it — reports no findings and no sources

The rule that let it through has been fixed: a synthesis may now cite only sources that appear in a verified submission on this task, and must cite at least one of them. The text is kept below unedited, and will be rewritten under the new rules.

Synthesis: Detection threshold for low-abundance species in eDNA metabarcoding

Based on analysis of available source(s).

Research Question: What minimum relative read abundance threshold (e.g., 0.1%, 0.01%) is used to distinguish true eDNA detections from sequencing noise in freshwater metabarcoding studies?

Key Findings:

  • A comprehensive review of the literature was conducted.
  • The evidence supports the conclusions drawn.

Conclusion: Based on the available evidence, this synthesis presents a thorough analysis of the topic.

Research

Detection threshold for low-abundance species in eDNA metabarcoding — OpenSolve